期刊
MOLECULAR AND CELLULAR BIOLOGY
卷 22, 期 2, 页码 442-452出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/MCB.22.2.442-452.2002
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资金
- NATIONAL CANCER INSTITUTE [R01CA048405, U01CA048405] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES [R37DK044746, R01DK044746] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM051104] Funding Source: NIH RePORTER
- NCI NIH HHS [CA48405, R01 CA048405] Funding Source: Medline
- NIDDK NIH HHS [R37 DK044746, DK44746] Funding Source: Medline
- NIGMS NIH HHS [GM51104] Funding Source: Medline
The replication initiation pattern of the murine beta -globin locus was analyzed in totipotent embryonic stem cells and in differentiated cell lines. Initiation events in the murine beta -globin locus were detected in a region extending from the embryonic Ey gene to the adult beta minor gene, unlike the restricted initiation observed in the human locus. Totipotent and differentiated cells exhibited similar initiation patterns. Deletion of the region between the adult globin genes did not prevent initiation in the remainder of the locus, suggesting that the potential to initiate DNA replication was not contained exclusively within the primary sequence of the deleted region. In addition, a deletion encompassing the six identified 5' hypersensitive sites in the mouse locus control region had no effect on initiation from within the locus. As this deletion also did not affect the chromatin structure of the locus, we propose that the sequences determining both chromatin structure and replication initiation lie outside the hypersensitive sites removed by the deletion.
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