Statistical optimization of culture conditions for enhanced bacterial cellulose production by Gluconoacetobacter hansenii NCIM 2529

In this study we carried out optimization of bacterial cellulose (BC) production from Gluconoacetobacter hansenii NCIM 2529 using a statistical model. Optimization of medium components and physical parameters used for bacterial cellulose production in G. hansenii NCIM 2529 was performed by two-step optimization. Eight culture parameters were screened by the Plackett-Burman design and significant parameters were optimized by the response surface methodology using a central composite design. Through these two-phase experiments, optimization of BC production was carried out for high production. The maximum average productivity of BC with optimized medium was 0.71 g/100 mL upon fermentation in a shake flask. The average BC production was observed to increase by about 44 % using these statistical techniques of media optimization.