期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 372, 期 1, 页码 216-220出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-001-1123-8
关键词
polyaromatic hydrocarbons; isotope dilution; high resolution mass spectrometry; human urine; solid-phase extraction
For measurement of biomarkers from polycyclic aromatic hydrocarbon (PAH) exposure, an analytical method is described quantifying hydroxylated PAH (OH-PAH) in urine samples. This method determined monohydroxy metabolites of naphthalene, fluorene, phenanthrene, fluoranthene, pyreno, chrysene, benzo[c]phenanthrene, and benz[a]anthracene. The sample preparation consisted of enzymatic hydrolysis, solid-phase extraction and derivatization with a silylating reagent. Five carbon-13 labeled standards were used for isotope dilution. Analytes were separated by gas chromatography (GC) and quantified with high-resolution mass spectrometry (HRMS). This method produced good recoveries (41-70%), linearity, and specificity. Data were corrected for blank levels from the naphthalene, fluorene, and phenanthrene metabolites. Method detection limits ranged from 2 ng L-1 for 1-hydroxypyrone to 43.5 ng L-1 for 1-hydroxynaphthalene. Using quality control charts from two urine pools, the method can be readily applied to biomonitoring PAH exposure.
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