期刊
CELLULAR SIGNALLING
卷 25, 期 11, 页码 2163-2175出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cellsig.2013.07.002
关键词
TRPM7; Aldosterone; Signal transduction; Mg2+
类别
资金
- Canadian Institute of Health Research (CIHR)
- National Institutes of Health [R01GM068801, R21AI0088421]
- NIGMS [R01GM90123]
- Office of Dietary Supplements, ODS [R01GM90123]
- Canada Research Chair/Canadian Foundation for Innovation award
- Heart and Stroke Foundation of Canada
- CIHR
- British Heart Foundation [RG/13/7/30099] Funding Source: researchfish
We demonstrated a role for the Mg2+ transporter TRPM7, a bifunctional protein with channel and alpha-kinase domains, in aldosterone signaling. Molecular mechanisms underlying this are elusive. Here we investigated the function of TRPM7 and its alpha-kinase domain on Mg2+ and pro-inflammatory signaling by aldosterone. Kidney cells (HEK-293) expressing wild-type human TRPM7 (WThTRPM7) or constructs in which the alpha-kinase domain was deleted (Delta Kinase) or rendered inactive with a point mutation in the ATP binding site of the alpha-kinase domain (K1648R) were studied. Aldosterone rapidly increased [Mg2+](i) and stimulated NADPH oxidase-derived generation of reactive oxygen species (ROS) in WT hTRPM7 and TRPM7 kinase dead mutant cells. Translocation of annexin-1 and calpain-II and spectrin cleavage (calpain target) were increased by aldosterone in WT hTRPM7 cells but not in alpha-kinase-deficient cells. Aldosterone stimulated phosphorylation of MAP kinases and increased expression of pro-inflammatory mediators ICAM-1, Cox-2 and PAL-1 in Delta kinase and K1648R cells, effects that were inhibited by eplerenone (mineralocorticoid receptor (MR) blocker). 2-APB, a TRPM7 channel inhibitor, abrogated aldosterone-induced Mg2+ responses in WT hTRPM7 and mutant cells. In 2-APB-treated Delta Kinase and K1648R cells, aldosterone-stimulated inflammatory responses were unchanged. These data indicate that aldosterone stimulates Mg2+ influx and ROS production in a TRPM7-sensitive, kinase-insensitive manner, whereas activation of annexin-1 requires the TRPM7 kinase domain. Moreover TRPM7 alpha-kinase modulates inflammatory signaling by aldosterone in a TRPM7 channel/Mg2+-independent manner. Our findings identify novel mechanisms for non-genomic actions of aldosterone involving differential signaling through MR-activated TRPM7 channel and alpha-kinase. (C) 2013 The Authors. Published by Elsevier Inc. All rights reserved.
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