期刊
EXPERIMENTAL NEUROLOGY
卷 174, 期 1, 页码 11-20出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1006/exnr.2001.7853
关键词
bone marrow; stem cell; transplantation; brain ischemia; stroke; middle cerebral artery occlusion; human; rat
资金
- NIAID NIH HHS [1F31AI/GM10291] Funding Source: Medline
- NIMH NIH HHS [1F30MH12157] Funding Source: Medline
- NINDS NIH HHS [R01-NS-40831] Funding Source: Medline
- NATIONAL INSTITUTE OF MENTAL HEALTH [F30MH012157] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE [R01NS040831] Funding Source: NIH RePORTER
There is now evidence to suggest that bone marrow mesenchymal stem cells (MSCs) not only differentiate into mesodermal cells, but can also adopt the fate of endodermal and ectodermal cell types. In this study, we addressed the hypotheses that human MSCs can differentiate into neural cells when implanted in the brain and restore sensorimotor function after experimental stroke. Purified human MSCs were grafted into the cortex surrounding the area of infarction 1 week after cortical brain ischemia in rats. Two and 6 weeks after transplantation animals were assessed for sensorimotor function and then sacrificed for histological examination. Ischemic rats that received human MSCs exhibited significantly improved functional performance in limb placement test. Histological analyses revealed that transplanted human MSCs expressed markers for astrocytes (GFAP(+)), oligodendroglia (GalC(+)), and neurons (betaIII(+), NF160(+), NF200(+), hNSE(+), and hNF70(+)). The morphological features of the grafted cells, however, were spherical in nature with few processes. Therefore, it is unlikely that the functional recovery observed by the ischemic rats with human MSC grafts was mediated by the integration of new neuronal cells into the circuitry of the host brain. The observed functional improvement might have been mediated by proteins secreted by transplanted hMSCs, which could have upregulated host brain plasticity in response to experimental stroke. (C) 2002 Elsevier Science (USA).
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