期刊
THERIOGENOLOGY
卷 57, 期 1, 页码 125-135出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/S0093-691X(01)00662-8
关键词
microfluidic; embryo; culture; maturation; livestock; bovine; in vitro production
The physical tools used in assisted reproduction have changed little over several decades. Microfluidics is an emerging technology that allows a fresh examination of the way assisted reproduction is performed. Here we review our work to develop microfluidic devices to perform the functions required in assisted reproduction. These functions include loading/unloading, culture, chemical manipulation, and mechanical manipulation of embryos and oocytes. Basic microfluidic theory and microfluidic device design and operation are discussed. Results are presented for mechanical removal of cumulus cells and for embryo culture, Results suggest that microfluidic systems will lead to improved efficiencies in assisted reproduction. (C) 2001 by Elsevier Science Inc.
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