期刊
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷 58, 期 3, 页码 416-420出版社
SPRINGER
DOI: 10.1007/s00253-001-0871-x
关键词
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The chromate-reducing ability of Pseudomonas aeruginosa A2Chr was compared in batch culture, with cells entrapped in a dialysis sac, and with cells immobilized in an agarose-alginate film in conjunction with a rotating biological contactor. In all three systems, the maximum Cr(VI) reduction occurred at 10 mg Cr(VI)/I. Whereas at 50 mg Cr(VI)/I concentration, only 16% of the total Cr(VI) was reduced, five spikings with 10 mg chromate/1 at 2-h, intervals led to 96% reduction of the total input of 50 mg Cr(VI)/1. Thus maximum Cr(VI) reduction was achieved avoidingCr(VI)toxicity to the cells by respiking with lower Cr(VI)concentrations. At 10 mg Cr(VI)/I, the pattern of chromate reduction in dialysis-entrapped cells was almost similar to that of batch culture and 86% of the bacterially reduced chromium was retained inside the dialysis sac. In electroplating effluent containing 100 mg Cr(VI)/I, however, the amount of Cr(VI) reduced by the cells immobilized in agarose-alginate biofilm was twice and thrice the amount reduced by batch culture and cells entrapped in a dialysis sac, respectively.
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