期刊
CLINICAL AND EXPERIMENTAL ALLERGY
卷 32, 期 3, 页码 391-396出版社
BLACKWELL PUBLISHING LTD
DOI: 10.1046/j.1365-2222.2002.01364.x
关键词
dendritic cell; ozone; sensitization; C57Bl/6 mice; airway inflammation
Background Epidemiological studies suggest that ozone exposure is related to increased asthma symptoms. Dendritic cells (DCs) are the principal antigen-presenting cells in the airways. Objective We have examined whether ambient doses of ozone (100 ppb for 2 h) enhance allergic sensitization and/or airway inflammation in a mouse model. Methods C57BL/6 mice were sensitized to inhaled ovalbumin (OVA) by intratracheal instillation of OVA-pulsed DCs on day 0. Daily exposure to OVA aerosol on days 14-20 resulted in an eosinophilic airway inflammation, as reflected in bronchoalveolar lavage fluid and lung histology. In a first experiment, mice were exposed to ozone or room air immediately prior to and following sensitization. Subsequently, we tested the effect of ozone exposure during antigen challenge in DC-sensitized mice. Results Exposure to ozone during sensitization did not influence airway inflammation after subsequent allergen challenge. In contrast, in sensitized mice, challenge with OVA together with ozone (days 14-20) resulted in enhanced airway eosinophilia and lymphocytosis, as compared with mice exposed to OVA and room air (1.91 x 10(6) +/- 0.46 x 10(6) vs. 0.16 x 10(6) +/- 0.06 x 10(6) eosinophils/mL lavage fluid; P = 0.0 15; 0.49 x 10(6) +/- 0.11 x 10(6) vs. 0.08 x 10(6) x 10(6) +/-0.03 x 10(6) lymphocytes/mL lavage fluid; P = 0.004). Ozone exposure without subsequent OVA exposure did not cause airway inflammation. Conclusion Ozone exposure does not increase allergic sensitization but enhances antigen-induced airway inflammation in mice that are sensitized via the airways.
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