期刊
JOURNAL OF CELLULAR BIOCHEMISTRY
卷 85, 期 3, 页码 583-591出版社
WILEY-BLACKWELL
DOI: 10.1002/jcb.10158
关键词
protein kinase CK2; nuclear matrix; heat shock; cancer cells; signaling
资金
- NATIONAL CANCER INSTITUTE [U01CA015062, R01CA015062, R37CA015062] Funding Source: NIH RePORTER
- NCI NIH HHS [CA-15062] Funding Source: Medline
Nuclear matrix, a key structure in the nuclear framework, appears to be a particularly responsive target during heat shock treatment of cells. We have previously shown that nuclear matrix is a preferential target for protein kinase CK2 signaling in the nucleus. The levels of CK2 in the nuclear matrix undergo dynamic changes in response to altered growth status in the cell. Here, we have demonstrated that CK2 targeting to the nuclear matrix is profoundly influenced by treatment of the cells to temperatures higher than 37degreesC. Rapid increase in the nuclear matrix association of CK2 is observed when cells are placed at temperatures of 41 and 45degreesC. This effect at 45 degreesC was higher than at 41 degreesC, and was time-dependent. Also, different cell lines behaved in a qualitatively similar manner though the quantitative responses differed. The modulations in the nuclear matrix associated CK2 in response to heat shock appear to be due to trafficking of the enzyme between cytosolic and nuclear compartments. In addition, it was noted that isolated nuclei subjected to heat shock also responded by a shuttling of the intrinsic CK2 to the nuclear matrix compartment. These results suggest that modulations in CK2 in the nuclear compartment in response to the heat stress occur not only by a translocation of the enzyme from the cytoplasmic compartment to the nuclear compartment, but also that there is a redistribution of the kinase within the nuclear compartment resulting in a preferential association with the nuclear matrix. The results support the notion that CK2 association with the nuclear matrix in response to heat shock may serve a protective role in the cell response to stress.
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