期刊
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
卷 28, 期 5, 页码 847-856出版社
KARGER
DOI: 10.1159/000335798
关键词
Red blood cells; Lysophosphatidic acid; Scramblase; Flippase; Calcium concentration; Phosphatidylserine; Fluorescence imaging
资金
- German Academic Exchange Service (DAAD)
- Ministry of Economy and Sciences of the Saarland
- European Community [FP7 2007-2013, NMP3-SL-2008-214032]
- DFG Graduate School [GRK 1276]
The exposure of phosphatidylserine (PS) on the outer membrane leaflet of red blood cells (RBCs) serves as a signal for eryptosis, a mechanism for the RBC clearance from blood circulation. The process of PS exposure was investigated as function of the intracellular Ca2+ content and the activation of PKC alpha in human and sheep RBCs. Cells were treated with lysophosphatidic acid (LPA), 4-bromo-A23187, or phorbol-12 myristate-13 acetate (PMA) and analysed by flow cytometry, single cell fluorescence video imaging, or confocal microscopy. For human RBCs, no clear correlation existed between the number of cells with an elevated Ca2+ content and PS exposure. Results are explained by three different mechanisms responsible for the PS exposure in human RBCs: (i) Ca2+-stimulated scramblase activation (and flippase inhibition) by LPA, 4-bromo-A23187, and PMA; (ii) PKC activation by LPA and PMA; and (iii) enhanced lipid flop caused by LPA. In sheep RBCs, only the latter mechanism occurs suggesting absence of scramblase activity. Copyright (C) 2011 S. Karger AG, Basel
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