期刊
PEPTIDES
卷 23, 期 1, 页码 31-42出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/S0196-9781(01)00576-9
关键词
crustacean hyperglycemic hormone; hydrothermal vents; MALDI-TOF mass spectrometry; X-organ-sinus gland complex; endoproteinase Lys-C
The structure of a well-known neurohormone involved in homeostasis regulation and stress response. the crustacean hyperglycemic hormone, was investigated in the deep-sea hydrothermal vent crab Bythograea thermlydron. The neuropeptide was isolated front neurohemal organs (sinus glands) and its biological activity checked using an homologous bioassay, Partial amino acid sequence was established by a combination of Edman chemistry and mass spectrometry. Then, the sequence of the cDNA encoding the hormone precursor was determined. The preprohormone(1) is composed of a 29 amino acid signal peptide, followed by a 41 amino acid associated peptide flanking the 72 amino acid hyperglycemic hormone. Comparison of these data with other known crab hyperglycemic hormone and prohormone sequences was per-formed using phylogenetic analysis methods. (C) 2002 Elsevier Science Inc. All rights reserved.
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