期刊
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
卷 282, 期 1, 页码 E177-E183出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajpendo.00316.2001
关键词
long-chain fatty acid; medium-chain fatty acid; malonyl-coenzyme A; carbohydrate; free fatty acid metabolism; free fatty acid oxidation; free fatty acid uptake
资金
- NATIONAL INSTITUTE OF ARTHRITIS AND MUSCULOSKELETAL AND SKIN DISEASES [R29AR045168] Funding Source: NIH RePORTER
- NIAMS NIH HHS [AR-45168] Funding Source: Medline
To determine whether changes in long-chain fatty acid (LCFA) oxidative metabolism induced by elevated intracellular carbohydrate availability are due to changes in LCFA uptake or in mitochondrial transport capacity, rat hindquarters were perfused with 500 muM palmitate and [1-C-14] palmitate or [1-C-14] octanoate as well as with either low (LG) or high (HG) carbohydrate availability. Glucose uptake was higher in the HG vs. LG group (23.6 +/- 1.5 vs 4.7 +/- 0.9 mu mol.g(-1).h(-1), P< 0.05). Palmitate delivery was not significantly different between groups and averaged 97.1 +/- 4.6 nmol.min(-1).g(-1) (P> 0.05). Fractional and total palmitate uptake values were 60% higher (P< 0.05) in the HG (0.125 +/- 0.012 and 7.4 +/- 1.2 nmol.min(-1).g(-1)) vs. LG (0.079 +/- 0.009 and 11.8 +/- 1.5 nmol.min(-1).g(-1)) group. Values of percent and total palmitate oxidized were significantly lower (P<0.05) in the HG (9.1 +/- 1.1% and 1.31 +/- 0.16 nmol.min(-1).g(-1)) vs. LG (23.4 +/- 5.2% and 0.76 +/- 0.08 nmol.min(-1).g(-1)) group. Conversely, values of fractional uptake and percent oxidation of octanoate were not significantly different between groups (P> 0.05). Malonyl-CoA levels were inversely correlated with LCFA oxidation (P< 0.05). These results demonstrate that high carbohydrate availability in muscle is associated with a decrease in LCFA oxidation that is not due to a parallel decrease in LCFA uptake; rather, the decrease in LCFA oxidation could be due to malonyl-CoA inhibition of mitochondrial LCFA transport.
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