期刊
CELLULAR MICROBIOLOGY
卷 14, 期 5, 页码 669-681出版社
WILEY-BLACKWELL
DOI: 10.1111/j.1462-5822.2012.01749.x
关键词
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资金
- Spanish Plan Nacional I+D+I grants [AGL2006-13503, AGL-2009-12757]
- Spanish Ministry of Science and Innovation [RYC-2007-01045]
- UPM-Bank Santander Sino
- National Science Foundation [IOS-1025642]
The bacterial pathogen Pseudomonas syringae pv tomato DC3000 suppresses plant innate immunity with effector proteins injected by a type III secretion system (T3SS). The cysteine protease effector HopN1, which reduces the ability of DC3000 to elicit programmed cell death in non-host tobacco, was found to also suppress the production of defence-associated reactive oxygen species (ROS) and callose when delivered by Pseudomonas fluorescens heterologously expressing a P. syringae T3SS. Purified His6-tagged HopN1 was used to identify tomato PsbQ, a member of the oxygen evolving complex of photosystem II (PSII), as an interacting protein. HopN1 localized to chloroplasts and both degraded PsbQ and inhibited PSII activity in chloroplast preparations, whereas a HopN1D299A non-catalytic mutant lost these abilities. Gene silencing of NtPsbQ in tobacco compromised ROS production and programmed cell death by DC3000. Our data reveal PsbQ as a contributor to plant immunity responses and a target for pathogen suppression.
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