期刊
CELLULAR MICROBIOLOGY
卷 15, 期 3, 页码 458-473出版社
WILEY-BLACKWELL
DOI: 10.1111/cmi.12050
关键词
-
资金
- US HIN [RE000954, DK020579, DK056341]
- Wellcome Trust UK [WT082825]
- Natural Sciences and Engineering Research Council of Canada
- Deutsche Forschungsgemeinschaft through the Collaborative Research Initiative 670 [SFB 670]
- Deutsche Forschungsgemeinschaft through Priority Programme [SPP1580]
- Studienstiftung des Deutschen Volkes
Rhodococcus equi is a close relative of Mycobacterium spp. and a facultative intracellular pathogen which arrests phagosome maturation in macrophages before the late endocytic stage. We have screened a transposon mutant library of R.equi for mutants with decreased capability to prevent phagolysosome formation. This screen yielded a mutant in the gene for -ketoacyl-(acyl carrier protein)-synthase A (KasA), a key enzyme of the long-chain mycolic acid synthesizing FAS-II system. The longest kasA mutant mycolic acid chains were 10 carbon units shorter than those of wild-type bacteria. Coating of non-pathogenic E.coli with purified wild-type trehalose dimycolate reduced phagolysosome formation substantially which was not the case with shorter kasA mutant-derived trehalose dimycolate. The mutant was moderately attenuated in macrophages and in a mouse infection model, but was fully cytotoxic.Whereas loss of KasA is lethal in mycobacteria, R.equi kasA mutant multiplication in broth was normal proving that long-chain mycolic acid compounds are not necessarily required for cellular integrity and viability of the bacteria that typically produce them. This study demonstrates a central role of mycolic acid chain length in diversion of trafficking by R.equi.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据