期刊
CELLULAR MICROBIOLOGY
卷 14, 期 2, 页码 239-+出版社
WILEY
DOI: 10.1111/j.1462-5822.2011.01715.x
关键词
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资金
- Cystic Fibrosis Canada
- Canadian Institutes of Health Research (CIHR)
- Heart and Stroke Foundation of Canada
- CCFF
- Canada Research Chair in Infectious Diseases and Microbial Pathogenesis
- Pitblado Chair in Cell Biology
Burkholderia cenocepacia, a member of the Burkholderia cepacia complex, is an opportunistic pathogen that causes devastating infections in patients with cystic fibrosis. The ability of B. cenocepacia to survive within host cells could contribute significantly to its virulence in immunocompromised patients. In this study, we explored the mechanisms that enable B. cenocepacia to survive inside macrophages. We found that B. cenocepacia disrupts the actin cytoskeleton of infected macrophages, drastically altering their morphology. Submembranous actin undergoes depolymerization, leading to cell retraction. The bacteria perturb actin architecture by inactivating Rho family GTPases, particularly Rac1 and Cdc42. GTPase inactivation follows internalization of viable B. cenocepacia and compromises phagocyte function: macropinocytosis and phagocytosis are markedly inhibited, likely impairing the microbicidal and antigen-presenting capability of infected macrophages. The type VI secretion system is essential for the bacteria to elicit these changes. This is the first report demonstrating inactivation of Rho family GTPases by a member of the B. cepacia complex.
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