期刊
CELLULAR MICROBIOLOGY
卷 10, 期 8, 页码 1695-1710出版社
WILEY
DOI: 10.1111/j.1462-5822.2008.01160.x
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资金
- FIC NIH HHS [D43-TW007129, D43 TW007129] Funding Source: Medline
- NCRR NIH HHS [5G12RR008124, G12 RR008124] Funding Source: Medline
- NHLBI NIH HHS [R01 HL059842, HL059842] Funding Source: Medline
- NIAID NIH HHS [R01 AI052733, R01 AI033142, AI52733, P30 AI051519, AI052733, AI056070-01A2, R37 AI033142, AI033142, R01 AI056070, R01 AI033774, R01 AI052733-05, R01 AI056070-04, AI51519, AI033774] Funding Source: Medline
Vesicular secretion of macromolecules has recently been described in the basidiomycete Cryptococcus neoformans, raising the question as to whether ascomycetes similarly utilize vesicles for transport. In the present study, we examine whether the clinically important ascomycete Histoplasma capsulatum produce vesicles and utilized these structures to secrete macromolecules. Transmission electron microscopy (TEM) shows transcellular secretion of vesicles by yeast cells. Proteomic and lipidomic analyses of vesicles isolated from culture supernatants reveal a rich collection of macromolecules involved in diverse processes, including metabolism, cell recycling, signalling and virulence. The results demonstrate that H. capsulatum can utilize a trans-cell wall vesicular transport secretory mechanism to promote virulence. Additionally, TEM of supernatants collected from Candida albicans, Candida parapsilosis, Sporothrix schenckii and Saccharomyces cerevisiae documents that vesicles are similarly produced by additional ascomycetes. The vesicles from H. capsulatum react with immune serum from patients with histoplasmosis, providing an association of the vesicular products with pathogenesis. The findings support the proposal that vesicular secretion is a general mechanism in fungi for the transport of macromolecules related to virulence and that this process could be a target for novel therapeutics.
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