期刊
CELLULAR IMMUNOLOGY
卷 257, 期 1-2, 页码 38-43出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.cellimm.2009.02.004
关键词
Nod2; Muramyl dipeptide; gamma delta T cells; Priming; Innate
资金
- NIH COBRE [P20 RR020185]
- NIH MAID [HHSN266200400009/NOI-AI40009]
- Murdock Charitable Trust
- NATIONAL CENTER FOR RESEARCH RESOURCES [P20RR020185] Funding Source: NIH RePORTER
Purified gamma delta T cells are primed directly in response to pathogen associated molecular patterns (PAMPs) to better respond to secondary signals and increase expression of chemokine and activation-related genes. Transcripts encoding the innate receptor Nod2 were detected in bovine and human gamma delta T cells. Nod2 is the intracellular receptor for muramyl dipeptide (MDP), functions in regulating innate activities, and was thought to be expressed primarily in APCs. The response of gamma delta T cells to MDP was analyzed by microarray, Q-PCR, proteome array and functional priming assays. MDP had a consistent priming effect on gamma delta T cells, characterized by changes in transcripts and enhanced proliferation response to secondary signaling. Knockdown experiments implicated Nod2 as the receptor for MDP in gamma delta T cell-enriched bovine PBLs. The results indicate priming of gamma delta T cells by MDP, and offer definitive evidence of the expression of functional Nod2 in gamma delta T cells. (C) 2009 Elsevier Inc. All rights reserved
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