期刊
CELLULAR IMMUNOLOGY
卷 253, 期 1-2, 页码 66-70出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.cellimm.2008.05.001
关键词
B cell; bone specific alkaline phosphatase; PMBC
We here report for the first time that human B cell express bone specific alkaline phosphatase. Using a monoclonal antibody against bone specific alkaline phosphatase (BAP), its expression was detected in approximately 50% of circulating B cells but not T cells. Using RT-PCR, BAP specific transcripts were detected in the B cells expressing surface BAP but not in those not expressing BAR Activation of B lymphocytes using phorbal ester, PMA with or without ionomycin did not affect change in BAP expression. Following stimulations with Pokeweed Mitogen (PWM) and Staphylococcus aureus Cowan I (SAC), BAP expression on human B cells was increased. This paralleled the increase in production of immunoglobulins and BAR detected by ELISA and enzyme assay with p-nitrophenyl phosphate, respectively. The role of BAP in B cell biology is uncertain. However, these data suggest that BAP may play an important role in differentiation and maturation of human B cells. (C) 2008 Elsevier Inc. All rights reserved.
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