4.5 Article

Ginkgo Biloba Extract (EGb 761) Promotes Peripheral Nerve Regeneration and Neovascularization After Acellular Nerve Allografts in a Rat Model

期刊

CELLULAR AND MOLECULAR NEUROBIOLOGY
卷 35, 期 2, 页码 273-282

出版社

SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s10571-014-0122-1

关键词

EGb 761; Acellular nerve allografts; Nerve regeneration; Angiogenesis

资金

  1. National High Technology Research and Development Program of China [2012AA020507]
  2. National Nature Science Grant of China [30700847]
  3. Combined Grant of Guangdong
  4. Ministry of Education of China [2007B090400090]
  5. Nature Science Grant of Guangdong China [9251008901000017]
  6. National Basic Research Program of China (973 Program) [2014CB542201]

向作者/读者索取更多资源

This study aimed to investigate whether or not ginkgo biloba extract (EGb 761) enhances peripheral nerve regeneration and vascularization after repair using acellular nerve allografts (ANA). Seventy-two Sprague-Dawley rats were randomly divided into three experimental groups: a unilateral 15-mm sciatic nerve defect was created and repaired with an autologous graft (autograft group); the same defect was repaired with an 18 mm ANA with an i.p. injection of normal saline for 10 days (saline group); and in the final group, the same defect was repaired with an 18 mm ANA with an i.p. injection of EGb 761 for 10 days (EGb 761 group). Axon outgrowth and vascularization were evaluated by immunocytochemistry 14 days post-implantation. The expression of genes associated with angiogenesis was analyzed by real-time polymerase chain reaction (PCR) seven days post-implantation. Compared with the saline group, rats in the EGb 761 group significantly increased the number of myelinated fibers and the average diameter of the nerves within the graft. There is no significant difference between the EGb 761 group and the autograft group. The expression of CD34 and NF200 was significantly higher in the EGb 761 group than in the saline group. Additionally, EGb 761 treatment increased the expression of several angiogenesis-related genes, including Vegf, SOX18, Prom 1, and IL-6. In conclusion, ANA repair with EGb 761 treatment demonstrates effects on peripheral nerve regeneration and vascularization that are equal to those of autologous graft repair, and that are superior to ANA repair alone.

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