期刊
CELLULAR AND MOLECULAR NEUROBIOLOGY
卷 34, 期 6, 页码 859-870出版社
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s10571-014-0063-8
关键词
Chitosan porous scaffold; Neural differentiation; Dental pulp stem cells (DPSCs); Brain injury; Biocompatibility
资金
- Graduate Student Innovation of Science and Technology Project in Jiangsu Province [YKC13084]
- Graduate Student Innovation of Science and Technology Project in Nantong University [YKC13084]
- Top Six Types of Talents Financial Assistance of Jiangsu Province Grant
- National Natural Science Foundation of China [31171038]
- Jiangsu Natural Science Foundation [BK2011385]
- Nantong University for Innovation Talent
- Priority Academic Program Development (PAPD) of Jiangsu Higher Education Institutions
A key aspect of cell replacement therapy in brain injury treatment is construction of a suitable biomaterial scaffold that can effectively carry and transport the therapeutic cells to the target area. In the present study, we created small 3D porous chitosan scaffolds through freeze-drying, and showed that these can support and enhance the differentiation of dental pulp stem cells (DPSCs) to nerve cells in vitro. The DPSCs were collected from the dental pulp of adult human third molars. At a swelling rate of similar to 4.33 +/- A 10.92 %, the scaffold displayed high porosity and interconnectivity of pores, as revealed by SEM. Cell counting kit-8 assay established the biocompatibility of the chitosan scaffold, supporting the growth and survival of DPSCs. The successful neural differentiation of DPSCs was assayed by RT-PCR, western blotting, and immunofluorescence. We found that the scaffold-attached DPSCs showed high expression of Nestin that decreased sharply following induction of differentiation. Exposure to the differentiation media also increased the expression of neural molecular markers Microtubule-associated protein 2, glial fibrillary acidic protein, and 2',3'-cyclic nucleotide phosphodiesterase. This study demonstrates that the granular 3D chitosan scaffolds are non-cytotoxic, biocompatible, and provide a conducive and favorable micro-environment for attachment, survival, and neural differentiation of DPSCs. These scaffolds have enormous potential to facilitate future advances in treatment of brain injury.
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