期刊
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
卷 25, 期 10-11, 页码 1651-1659出版社
MARCEL DEKKER INC
DOI: 10.1081/JLC-120005712
关键词
orange essential oils; monoterpenes; capillary LC; MEKC
This paper describes the evaluation of alternative techniques, for the determination of selected volatile compounds in cold-pressed orange oil. Two techniques were investigated: capillary-LC (C-LC) and micellar electrokinetic chromatography (MEKC). The separation by C-LC was performed on a 23 cm. x 0.53 mm i.d. fused-silica column packed in house with RP-18. Acetonitrile:water (80:20) was used as mobile phase. The compounds were detected at 195 and 240 nm in a UV-detector with either a Z or U shaped flow cell. In MEKC, 50 mM SDS was used in a 20 mM phosphate buffer at pH 8.1, and separations were carried out at 20 kV A fused-silica capillary (75 mum i.d., 52.5 cm effective length, 60 cm total length) was used. On-column UV detection was conducted at 214 and 254 nm. A clean-up with C-18 as adsorbent and acetonitrile as eluent was performed before the chromatographic and electrophoretic runs in order to remove the colored components that could promote interference in the analysis. Capillary-LC allowed the determination of terpenes such as mircene, limonene, alpha-pinene, and oxygenated terpenes including linalool, citral, and carvone while, in MEKC, carvone, mircene, linalool, limonene, and citral were determined.
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