期刊
CELLULAR AND MOLECULAR NEUROBIOLOGY
卷 29, 期 6-7, 页码 1037-1043出版社
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s10571-009-9393-3
关键词
Motor neurons; Amyotrophic lateral sclerosis; Glial cells; Valproic acid; Kainate; Excitotoxicity; Calcium-imaging
资金
- Deutsche Gesellschaft fur Muskelkranke (DGM)
Chronic dysregulation of the intracellular Ca(2+) homeostasis (excitotoxicity) is thought to contribute to the development of motor neuron diseases. Valproic acid (VPA) is widely used as an antiepileptic drug and acts mainly by inhibition of sodium channels and by enhancing the level of the inhibitory neurotransmitter gamma-aminobutyric acid. Neuroprotective capacities of VPA are supposed to arise also from the inhibition of histone deacetylases. We investigated the viability of highly purified rat embryonic motor neurons cultured on glial feeder layers, composed of either astrocytes or Schwann cells, or in the absence of glia, monoculture in presence of VPA and/or kainate (KA) using immunocytochemistry and calcium imaging. A significant effect of the culture and co-culture conditions on the viability of motor neurons in our in vitro model of excitotoxicity was detected. The neuroprotective effect of VPA on primary embryonic motor neuron cultures was not proven. A functional interaction between VPA and KA occurred during the first 10 days in culture.
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