期刊
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
卷 50, 期 3, 页码 437-439出版社
HISTOCHEMICAL SOC INC
DOI: 10.1177/002215540205000315
关键词
autofluorescence; photobleaching; immunofluorescence
类别
Autofluorescence of aldehyde-fixed neural tissue often complicates the use of fluorescence microscopy. Background fluorescence can be notably reduced or eliminated by irradiation with light before treatment with fluorescence probes, resulting in a higher contrast without adversely affecting the staining probabilities.
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