期刊
GENESIS
卷 32, 期 3, 页码 199-202出版社
WILEY-LISS
DOI: 10.1002/gene.10030
关键词
gene targeting; deleter; transgene; Cre recombinase; Hprt; transgene
资金
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM048103] Funding Source: NIH RePORTER
- NIGMS NIH HHS [2R01GM48103-04] Funding Source: Medline
A Cre recombinase expression cassette was inserted into the X-linked Hprt locus by gene targeting in a mouse embryonic stem (ES) cell line isogenic to strain 129S1/SvImJ (129S1), then the transgene was introduced into 129S1 mice through ES cell chimeras. When females hemizygous for this transgene were mated to males carrying a neomycin selection cassette flanked by loxP sites, the cassette was always excised regardless of Cre inheritance and without detectable mosaicism. The usefulness of this Cre-deleter transgenic line is in its efficiency and defined genetic status in terms of mouse strain and location of the transgene. genesis 32: 199-202, 2002. (C) 2002 Wiley-Liss, Inc.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据