期刊
CELLULAR AND MOLECULAR LIFE SCIENCES
卷 71, 期 2, 页码 311-329出版社
SPRINGER BASEL AG
DOI: 10.1007/s00018-013-1375-4
关键词
Paraspeckles; Nucleolar caps; Annexin A10; Apoptosis
资金
- Interdisciplinary Clinical Research Centre of the University of Munster (IZKF) [RE2/017/10]
- German Research Foundation (DFG) [RE2611/2-1, SFB 629/A1]
Annexin A10 is the latest identified member of the annexin family of Ca2+- and phospholipid-binding proteins. In previous studies, downregulation of annexin A10 was correlated with dedifferentiation, invasion, and tumor progression, pointing to a possible tumor suppressor role. However, the biochemical characteristics and functions of annexin A10 remain unknown. We show that annexin A10 displays biochemical characteristics atypical for an annexin, indicating a Ca2+- and membrane-binding-independent function. Annexin A10 co-localizes with the mRNA-binding proteins SFPQ and PSPC1 at paraspeckles, an only recently discovered nuclear body, and decreases paraspeckle numbers when overexpressed in HeLa cells. In addition, annexin A10 relocates to dark perinucleolar caps upon transcriptional inhibition of RNA polymerase II. We mapped the cap-binding function of annexin A10 to the proximal part of the core domain, which is missing in the short isoform of annexin A10, and show its independence from the remaining functional type II Ca2+-binding site. In contrast to this, paraspeckle recruitment required additional core regions and was negatively affected by the mutation of the last type II Ca2+-binding site. Additionally, we show that overexpression of annexin A10 in HeLa cells increases their sensitivity to apoptosis and reduces colony formation. The identification of unique nuclear and biochemical characteristics of annexin A10 points towards its membrane-independent role in paraspeckle-associated mRNA regulation or processing.
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