期刊
CELLULAR AND MOLECULAR LIFE SCIENCES
卷 70, 期 2, 页码 357-371出版社
SPRINGER BASEL AG
DOI: 10.1007/s00018-012-1114-2
关键词
CCT; Microtubule dynamics; +TIPs; Tubulin folding cofactors
资金
- Consolider-Ingenio Spanish Ministry of Education and Science Centrosome-3D
- Instituto de Formacion e Investigacion Marques de Valdecilla
- [BFU2007-64882]
- [BFU2008-01344]
- [BFU2010-18948]
Tubulin cofactors (TBCs) participate in the folding, dimerization, and dissociation pathways of the tubulin dimer. Among them, TBCB and TBCE are two CAP-Gly domain-containing proteins that together efficiently interact with and dissociate the tubulin dimer. In the study reported here we showed that TBCB localizes at spindle and midzone microtubules during mitosis. Furthermore, the motif DEI/M-COO- present in TBCB, which is similar to the EEY/F-COO- element characteristic of EB proteins, CLIP-170, and alpha-tubulin, is required for TBCE-TBCB heterodimer formation and thus for tubulin dimer dissociation. This motif is responsible for TBCB autoinhibition, and our analysis suggests that TBCB is a monomer in solution. Mutants of TBCB lacking this motif are derepressed and induce microtubule depolymerization through an interaction with EB1 associated with microtubule tips. TBCB is also able to bind to the chaperonin complex CCT containing alpha-tubulin, suggesting that it could escort tubulin to facilitate its folding and dimerization, recycling or degradation.
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