期刊
CELLULAR AND MOLECULAR LIFE SCIENCES
卷 70, 期 5, 页码 815-828出版社
SPRINGER BASEL AG
DOI: 10.1007/s00018-012-1081-7
关键词
Transgenic technologies; Gene targeting; RNA interference; Induced pluripotent stem cells
资金
- Major Science and Technology Project of New Variety Breeding of Genetically Modified Organisms [2009ZX08008-004B, 2008ZX08008-003]
- National High Technology Research Development Program of China (863 Program) [2008AA10Z140]
- National Natural Science Foundation of China [30571339]
- Innovation Research Foundation of CAAS [2004-CAAS-1]
Transgenic animal technology is one of the fastest growing biotechnology areas. It is used to integrate exogenous genes into the animal genome by genetic engineering technology so that these genes can be inherited and expressed by offspring. The transgenic efficiency and precise control of gene expression are the key limiting factors in the production of transgenic animals. A variety of transgenic technologies are available. Each has its own advantages and disadvantages and needs further study because of unresolved technical and safety issues. Further studies will allow transgenic technology to explore gene function, animal genetic improvement, bioreactors, animal disease models, and organ transplantation. This article reviews the recently developed animal transgenic technologies, including the germ line stem cell-mediated method to improve efficiency, gene targeting to improve accuracy, RNA interference-mediated gene silencing technology, zinc-finger nuclease gene targeting technology and induced pluripotent stem cell technology. These new transgenic techniques can provide a better platform to develop transgenic animals for breeding new animal varieties and promote the development of medical sciences, livestock production, and other fields.
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