期刊
CELLULAR AND MOLECULAR LIFE SCIENCES
卷 68, 期 19, 页码 3219-3232出版社
SPRINGER BASEL AG
DOI: 10.1007/s00018-011-0627-4
关键词
SUMO (small ubiquitin-related modifier); Ubiquitin; Cell stress; Monoubiquitylated histone 2B; Development; Gene expression; Muscle; C. elegans
资金
- Academy of Finland
- Finnish Cancer Organisations
- Sigrid Juselius Foundation
- Saastamoinen Foundation
- Biocenter Finland
- Kuopio Graduate School of Molecular Medicine
Small ubiquitin-related modifiers (SUMOs) are important regulator proteins. Caenorhabditis elegans contains a single SUMO ortholog, SMO-1, necessary for the reproduction of C. elegans. In this study, we constructed transgenic C. elegans strains expressing human SUMO-1 under the control of pan-neuronal (aex-3) or pan-muscular (myo-4) promoter and SUMO-2 under the control of myo-4 promoter. Interestingly, muscular overexpression of SUMO-1 or -2 resulted in morphological changes of the posterior part of the nematode. Movement, reproduction and aging of C. elegans were perturbed by the overexpression of SUMO-1 or -2. Genome-wide expression analyses revealed that several genes encoding components of SUMOylation pathway and ubiquitin-proteasome system were upregulated in SUMO-overexpressing nematodes. Since muscular overexpression of SMO-1 also brought up reproductive and mobility perturbations, our results imply that the phenotypes were largely due to an excess of SUMO, suggesting that a tight control of SUMO levels is important for the normal development of multicellular organisms.
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