期刊
CELLULAR AND MOLECULAR LIFE SCIENCES
卷 69, 期 2, 页码 267-282出版社
SPRINGER BASEL AG
DOI: 10.1007/s00018-011-0652-3
关键词
Myogenesis; Visceral mesoderm; Myoblast; Muscle attachment; Extracellular matrix; ECM; kon-tiki; thrombospondin
资金
- Deutsche Forschungsgemeinschaft [Ho-2559/3-2, 3-3]
In Drosophila, four genes encode for laminin subunits and the formation of two laminin heterotrimers has been postulated. We report the identification of mutations in the Drosophila LamininB2 (LanB2) gene that encodes for the only laminin gamma subunit and is found in both heterotrimers. We describe their effects on embryogenesis, in particular the differentiation of visceral tissues with respect to the ECM. Analysis of mesoderm endoderm interaction indicates disrupted basement membranes and defective endoderm migration, which finally interferes with visceral myotube stretching. Extracellular deposition of laminin is blocked due to the loss of the LanB2 subunit, resulting in an abnormal distribution of ECM components. Our data, concerning the different function of both trimers during organogenesis, suggest that these trimers might act in a cumulative way and probably at multiple steps during ECM assembly. We also observed genetic interactions with kon-tiki and thrombospondin, indicating a role for laminin during muscle attachment.
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