期刊
CELL AND TISSUE RESEARCH
卷 307, 期 1, 页码 69-77出版社
SPRINGER
DOI: 10.1007/s004410100454
关键词
surfactant subtype; alkaline phosphatase isozyme; surfactant-like particle protein; lipopolysaccharide; bronchoalveolar fluid; rat [Crj : CD(SD)IGS]
类别
The characteristics of lipopolysaccharide (LPS)induced alkaline phosphatase (AP) isozymes on the various pulmonary surfactant subtypes were investigated. We used continuous sucrose-gradient centrifugation to separate surfactant into subtypes. The density of each surfactant subtype isolated from LPS-instilled rats was greater than that of the subtypes from the control rats; and the proportion of light surfactant was lower, thereby decreasing the ratio of light to heavy surfactant. The results of an inhibition study revealed the main AP isozyme in bronchoalveolar fluid (BAF) to be tissue nonspecific AP (TNAP), but some of the activity was characteristic of intestinal-type AP (IAP). IAP. in addition to TNAP and surfactant-associated protein A (SPA), was detected on heavy surfactant, and LPS induced both APs. To examine the expression of IAP in the lungs, we prepared primers to detect the cDNAs of two types of rat IAP mRNA, IAP-I and -II, and amplified their cDNAs. LPS instillation induced IAP-I mRNA, but not IAP-II mRNA or TNAP mRNA. Immunohistochemical localization of IAP and TNAP revealed reaction products for both in type II cells. The present study thus demonstrated that, in rats, type II cells produce both IAP and TNAP and that these surfactants bearing AP isozymes are secreted into the alveolar space following induction by intratracheal instillation of LPS.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据