期刊
CELLULAR AND MOLECULAR LIFE SCIENCES
卷 68, 期 9, 页码 1593-1610出版社
BIRKHAUSER VERLAG AG
DOI: 10.1007/s00018-010-0535-z
关键词
Cell polarity; Centrosome; Cytoskeleton; KASH-domain; Kinesin-1; Lamin A/C; Laminopathies; Nesprin
资金
- Deutsche Forschungsgemeinschaft [KA 2778/1-1]
- St. Moritz-Foundation
- Wellcome Trust
Cell polarization is a fundamental process underpinning organismal development, and tissue homeostasis, which requires an orchestrated interplay of nuclear, cytoskeletal, and centrosomal structures. The underlying molecular mechanisms, however, still remain elusive. Here we report that kinesin-1/nesprin-2/SUN-domain macromolecular assemblies, spanning the entire nuclear envelope (NE), function in cell polarization by anchoring cytoskeletal structures to the nuclear lamina. Nesprin-2 forms complexes with the kinesin-1 motor protein apparatus by associating with and recruiting kinesin light chain1 (KLC1) to the outer nuclear membrane. Similar to nesprin-2, KLC1 requires lamin A/C for proper NE localization. The depletion of nesprin-2 or KLC1, or the uncoupling of nesprin-2/SUN-domain protein associations impairs cell polarization during wounding and dislodges the centrosome from the NE. In addition nesprin-2 loss has profound effects on KLC1 levels, the cytoskeleton, and Golgi apparatus organization. Collectively these data show that NE-associated proteins are pivotal determinants of cell architecture and polarization.
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