期刊
CELLULAR AND MOLECULAR LIFE SCIENCES
卷 67, 期 23, 页码 4065-4077出版社
SPRINGER BASEL AG
DOI: 10.1007/s00018-010-0426-3
关键词
Immediate-early genes; Chromatin structure; Factor binding; Histone deacetylases; Histone acetyltransferases
资金
- Ministry of Science and Innovation (Spain) [BFU2007-63120, CSD2006-49]
- Generalitat Valenciana [ACOMP07/085]
- Ministry of Education (Spain) [BMC 2001-2868]
The influence of chromatin on immediate-early gene expression has been studied in a model of Egr1 induction in intact mouse cells. ChIP analysis of factor and RNA polymerase binding reveals that the gene is constitutively poised for transcription in nonstimulated cells, but a repressing chromatin structure hampers productive transcription. Stimulation with phorbol esters results in a transient activation, which starts at 5 min and peaks at 30 min. Quantitative mapping of promoter occupancy by the different factors shows for the first time that no direct competition between SP1 and EGR1 occurs. The phosphorylation of ELK1 and CREB, which involves both the cascades of MEK1/2 and p38 kinases, is required for gene expression, which ceases following the binding of NAB1 and NAB2 to the promoter. The changes in histone acetylation and the differential recruitment of histone-modifying complexes further show the role of chromatin in the activation of this immediate-early gene.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据