期刊
CELLULAR AND MOLECULAR LIFE SCIENCES
卷 67, 期 7, 页码 1189-1202出版社
SPRINGER BASEL AG
DOI: 10.1007/s00018-009-0247-4
关键词
Chimera; ES cell; Host accessibility; gamma-Irradiation; Medaka
资金
- Biomedical Research Council of Singapore [R-08-1-21-19-585, R-154-000-371-305, SBIC-SSCC C-002-2007]
- Ministry of Education of Singapore [R-154-000285-112]
- Lee Hiok Kwee donation fund [R-154-000-153-720]
- Deutsche Forschungsgemeinschaft
- Commission of the European Union
Chimera formation is a powerful tool for analyzing pluripotency in vivo. It has been widely accepted that host cell lineages are generally accessible to embryonic stem (ES) cells with the actual contribution depending solely on the intrinsic pluripotency of transplanted donor cells. Here, we show in the fish medaka (Oryzias latipes) that the host accessibility to ES cell contribution exhibits dramatic differences. Specifically, of three albino host strains tested (i (1) , i (3) and af), only strain i (1) generated pigmented chimeras. Strikingly, this accessibility is completely lost in i (1) but acquired in i (3) after host gamma-irradiation. Host irradiation also differentially affected ES cell contribution to somatic organs and gonad. Therefore, the accessibility of various host cell lineages can vary considerably depending on host strains and cell lineages as well as on irradiation. Our findings underscore the importance of host genotypes for interpreting donor cell pluripotency and for improving ES-derived chimera production.
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