期刊
BIOELECTROCHEMISTRY
卷 55, 期 1-2, 页码 13-15出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/S1567-5394(01)00160-8
关键词
enzyme immobilisation; poly(aniline) composite films; NADH; biofuel cell
Immobilisation of enzymes is important for applications such as biosensors or biofuel cells. A poly(histidine) tag had been introduced on the C terminus of a lactate dehydrogenase enzyme. This mutant enzyme was then immobilised onto poly(aniline) (PANi)-poly(anion) composite films, PANi-poly(vinylsulfonate) (PVS) or PANi-poly(acrylate) (PAA). The NADH produced by the immobilised enzyme in the presence of beta-nicotinamide adenine dinucleotide (NAD+) and lactate is oxidised at the poly(aniline)-coated electrode at 0.05 to 0.1 V vs. saturated calomel electrode (SCE) at 35 degreesC. (C) 2002 Elsevier Science B.V All rights reserved.
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