期刊
CELLULAR AND MOLECULAR BIOENGINEERING
卷 7, 期 4, 页码 544-551出版社
SPRINGER
DOI: 10.1007/s12195-014-0358-3
关键词
Laminopathy; Metastasis; Cell mechanics
资金
- NIH [CA-125707]
- NSF [CBET 0954421 CAREER]
During metastasis, melanoma cells must be sufficiently deformable to squeeze through extracellular barriers with small pore sizes. We visualize and quantify deformability of single cells using micropipette aspiration and examine the migration potential of a population of melanoma cells using a flow migration apparatus. We artificially stiffen the nucleus with recombinant overexpression of Delta 50 lamin A, which is found in patients with Hutchison Gilford progeria syndrome and in aged individuals. Melanoma cells, both WM35 and Lu1205, both show reduced nuclear deformability and reduced cell invasion with the expression of Delta 50 lamin A. These studies suggest that cellular aging including expression of Delta 50 lamin A and nuclear stiffening may reduce the potential for metastatic cancer migration. Thus, the pathway of cancer metastasis may be kept in check by mechanical factors in addition to known chemical pathway regulation.
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