期刊
VIRUS RESEARCH
卷 97, 期 2, 页码 81-87出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0168-1702(03)00222-3
关键词
bovine leukemia virus; bovine immunodeficiency-like virus; cattle; lymphocyte; PCR
类别
Cellular tropism and transcription of bovine leukemia virus (BLV) and bovine immunodeficiency-like virus (BIV) were investigated using peripheral blood mononuclear cells (PBMC) collected from a cow infected with both viruses. Each PBMC subset, purified by magnetic cell sorting, was subjected to PCR and RT-PCR for detection of their integrated proviruses and transcript mRNAs. Both BLV and BIV genomes were detected by nested PCR in CD3(+), CD4(+), CD8(+) and gammadelta T cells, B cells and monocytes. However, BLV tax transcription was only detected in B cells. and only B cells also formed BLV syncytia in CC81 cells. On the other hand, BIV transcript was detected in each subpopulation of PBMC. These results indicated that BLV can infect T cells and monocytes as well as B cells, but can be expressed by transcription only in B cells. In contrast. BIV can express its transcripts in all infected cells. (C) 2003 Elsevier B.V. All rights reserved.
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