期刊
BIOCHEMICAL ENGINEERING JOURNAL
卷 16, 期 3, 页码 287-297出版社
ELSEVIER
DOI: 10.1016/S1369-703X(03)00072-X
关键词
Phaeodactylum tricomutum; microalgal culture; photobioreactors; airlift reactors
A bubble column and two airlift photobioreactors (a draft-tube sparged vessel and a split-cylinder device) of the same general design (0.19 m column diameter, 2 m tall, 0.06 m(3) working volume) were evaluated for outdoor continuous culture of the microalga Phaeodactylum tricomutum at a dilution rate of 0.03 h(-1). At a daily averaged irradiance (photosynthetically active) value of 900 muE m(-2) s(-1), all bioreactors attained a quasi steady-state biomass concentration of similar to1 kg m(-3) and a biomass productivity of similar to0.3 kg m(-3) per day when the aeration velocity was 0.01 m s(-1). The microalgal cells were susceptible to aeration-associated hydrodynamic stress if the superficial aeration velocity exceeded 0.01 m s(-1). Supplementing the culture medium with 0.02% or more carboxymethyl cellulose (CMC), allowed stable culture under conditions that had previously damaged the cells. The average elemental composition of the biomass was: 49.2% C, 6.3% H, 0.8% N, and 1.3% S. The chlorophylls, carotenoids, and pigments content of the biomass changed with irradiance within a given day. Low irradiance favored accumulation of the light capture pigments. Increasing daily irradiance led to accumulation of carbohydrates. Some of the carbohydrate accumulated during the day was consumed at night and partly converted to proteins. Eicosapentaenoic acid (EPA, 20:5n3) constituted between 27 and 30% of the total fatty acids present, or 2.6-3.1% of the dry biomass. The other main fatty acids present were palmetic acid (16:0), palmoleic acid (16: 10), and myristic acid (14:0). On average, these three fatty acids constituted 16.9% (16:0), 14.0% (16:1n7) and 9.4% (14:0) of the total fatty acids present. (C) 2003 Elsevier Science B.V. All rights reserved.
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