期刊
CELLS TISSUES ORGANS
卷 197, 期 1, 页码 14-26出版社
KARGER
DOI: 10.1159/000339772
关键词
Actin cytoskeleton; Biomaterial; Cartilage, articular; Cartilage research; Cell-cell and cell-matrix interactions; Cytoskeleton; Cytoskeleton interaction; Tissue engineering, cartilage and bone
资金
- UNL-Tobacco Settlement Funds
- NIH [R21EB006046]
- Department of Health and Human Services [1R21RR024437-01A1]
The impact of low-intensity diffuse ultrasound (LIDUS) stimulation on the cytoskeletal organization of chondrocytes seeded in three-dimensional (3D) scaffolds was evaluated. Chondrocytes seeded on 3D chitosan matrices were exposed to LIDUS at 5.0 MHz (approx. 15 kPa, 51 s, 4 applications/day) in order to study the organization of actin, tubulin and vimentin. The results showed that actin presented a punctate cytosolic distribution and tubulin presented a quasiparallel organization of microtubules, whereas vimentin distribution was unaffected. Chondrocytes seeded on 3D scaffolds responded to US stimulation by the disruption of actin stress fibers and were sensitive to the presence of Rho-activated kinase (ROCK) inhibitor (Y27632). The gene expression of ROCK-I, a key element in the formation of stress fibers and mDia1, was significantly upregulated under the application of US. We conclude that the results of both the cytoskeletal analyses and gene expression support the argument that the presence of punctate actin upon US stimulation was accompanied by the upregulation of the RhoA/ROCK pathway. Copyright (C) 2012 S. Karger AG, Basel
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