期刊
CELLS TISSUES ORGANS
卷 190, 期 3, 页码 135-149出版社
KARGER
DOI: 10.1159/000187716
关键词
Cord blood; Stem cell, unrestricted somatic; Poly(epsilon-caprolactone); Nanofiber scaffold; Hepatocyte differentiation
资金
- Stem Cell Technology Company
Tissue engineering of implantable cellular constructs is an emerging cellular therapy for hepatic disease. In this study, we tested the ability of poly(epsilon-caprolactone) (PCL) nanofiber scaffold to support and maintain hepatic differentiation of human cord blood-derived unrestricted somatic stem cells (USSCs) in vitro. USSCs, self-renewing pluripotent cells, were isolated from human cord blood. The electrospun PCL nanofiber porous scaffold was constructed of uniform, randomly oriented nanofibers. USSCs were seeded onto PCL nanofiber scaffolds, and were induced to differentiate into hepatogenic lineages by culturing with differentiation factors for 6 weeks. RT-PCR analysis of endoderm and hepatic-specific gene expression, immunohistochemical detection of cytokeratin 18 (CK-18), alpha-fetoprotein, albumin, glycogen storage and in docyanine green uptake confirmed the differentiation of USSCs into endoderm and hepatocyte-like cells. In the present study, we show that hepatocyte-like cells differentiated from USSCs on the PCL nanofiber scaffold can be candidate for tissue engineering and cell therapy of hepatic tissues. Copyright (C) 2008 S. Karger AG, Basel
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