期刊
TRENDS IN BIOTECHNOLOGY
卷 21, 期 1, 页码 45-52出版社
ELSEVIER SCIENCE LONDON
DOI: 10.1016/S0167-7799(02)00006-9
关键词
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Cell-surface display allows peptides and proteins to be displayed on the surface of microbial cells by fusing them with the anchoring motifs. The protein to be displayed - the passenger protein - can be fused to an anchoring, motif - the carrier protein - by N-terminal fusion, C-terminal fusion or sandwich fusion. The characteristics of carrier protein, passenger protein and host cell, and fusion method all affect the efficiency of surface display of proteins. Microbial cell-surface display has many potential applications, including live vaccine development, peptide library screening, bioconversion using whole cell biocatalyst and bioadsorption.
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