期刊
ENVIRONMENTAL MICROBIOLOGY
卷 5, 期 1, 页码 72-74出版社
BLACKWELL PUBLISHING LTD
DOI: 10.1046/j.1462-2920.2003.00381.x
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Building materials commonly used in wall paintings and monuments (mortar, limestone and sandstone) were inoculated with an artificial consortium composed of 14 microorganisms and incubated for 6 months at 28degreesC. The colonization of the different materials by the consortium was investigated. Culture-independent techniques revealed the presence of a diversity of bacteria, whereas culture-dependent techniques yielded mainly spore-forming bacteria. The data suggest that plating leads to an overestimation of the number of spore-forming bacteria with respect to quiescent vegetative forms; the latter are less easily cultured, but are readily detected by culture-independent techniques.
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