3.8 Article

Mapping and QTL analysis of the barley population Sloop x Halcyon

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AUSTRALIAN JOURNAL OF AGRICULTURAL RESEARCH
卷 54, 期 11-12, 页码 1145-1153

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C S I R O PUBLISHING
DOI: 10.1071/AR03037

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linkage map; molecular markers; disease resistance; phenology; grain colour; validation

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A genetic linkage map of Hordeum vulgare L. 1280 cM in length, composed of 257 AFLP, RFLP, SNP, and microsatellite markers, has been constructed. The map was based on a doubled haploid population made from the cross Sloop ( spring type) x Halcyon ( winter type). The genetic map was used to identify qualitative major genes and quantitative trait loci ( QTLs) affecting traits related to growth and flowering, grain colour, and disease resistance. Nine QTLs associated with grain colour ( brightness, redness, yellowness, blue aleurone colour), plant height, ' intrinsic lateness', awn emergence, response to photoperiod, and spring or winter habit were located on 1H, 2H, 3H, 4H, and 5H. Eight QTLs associated with resistance to scald, net form of net blotch, leaf rust and powdery mildew were identified on chromosomes 1H, 2H, 3H, 4H, 5H, and 7H. The estimated magnitude of the QTL effects ranged from 9 to 85% of the total phenotypic variance. Resistances to leaf scald, net blotch, and leaf rust, and photoperiod and grain colour, were each controlled by at least one major gene.

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