期刊
CELL TRANSPLANTATION
卷 18, 期 12, 页码 1369-1379出版社
SAGE PUBLICATIONS INC
DOI: 10.3727/096368909X474230
关键词
Human mesenchymal stem cell (hMSC); Stroke; Brain ischemia; Cell transplantation; Biodistribution; Nuclear imaging
资金
- INSERM
- Association Nationale de la Recherche Technique (ANRT)
- INSERM/DHOS
- J. Fourier Grenoble University UJF-Vivier de la Recherche Medicale
Human mesenchymal stein cells (hMSC) are a promising Source for cell therapy after stroke. To deliver these cells, in IV injection appears safer than a local graft. We aimed to assess the whole-body biodistribution of IV-injected Tc-99m-HMPAO-labeled hMSC in normal rats (n = 9) and following a right middle cerebral artery occlusion (MCAo, n = 9). Whole-body nuclear imaging, isolated organ Counting (at 2 and 20 h after injection) and histology were performed. A higher activity was observed in the right damaged hemisphere of the MCAo group [6.5 +/- 0.9 x 10(-3) % of injected dose (ID)/g] than in the control group (3.6 +/- 1.2 x 10(-3) %ID/g), 20 h after injection. In MCAo rats, right hemisphere activity was higher than that observed in the contralateral, hemisphere at 2 h after injection (11.6 +/- 2.8 vs. 9.8 +/- 1.7 x 10-3 %ID/g). Following an initial hMSC lung accumulation, there was a decrease in pulmonary activity from 2 to 20 h after injection in both groups. The spleen was the only organ in which activity increased between 2 and 20 h. The presence of hMSC was documented in the spleen, liver, king, and brain following histology. IV-injected hMSC are transiently trapped in the lungs, can be sequestered in the spleen, and are predominantly eliminated by kidneys. After 20 h, more hMSC are found in the ischemic lesion than into the undamagged cerebral tissue. IV delivery of hMSC could be the initial route for a clinical trial of tolerance.
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