期刊
CELL STRESS & CHAPERONES
卷 20, 期 1, 页码 85-93出版社
SPRINGER
DOI: 10.1007/s12192-014-0528-1
关键词
Exercise; HSP70; Intestinal permeability
类别
资金
- University of New Mexico's Research Allocation Committee
- National Center for Advancing Translational Sciences of the National Institutes of Health [UL1 TR000041]
- NATIONAL CENTER FOR ADVANCING TRANSLATIONAL SCIENCES [UL1TR000041] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES [P30DK072488] Funding Source: NIH RePORTER
Chronic glutamine supplementation reduces exercise-induced intestinal permeability and inhibits the NF-kappa B pro-inflammatory pathway in human peripheral blood mononuclear cells. These effects were correlated with activation of HSP70. The purpose of this paper is to test if an acute dose of oral glutamine prior to exercise reduces intestinal permeability along with activation of the heat shock response leading to inhibition of pro-inflammatory markers. Physically active subjects (N = 7) completed baseline and exercise intestinal permeability tests, determined by the percent ratio of urinary lactulose (5 g) to rhamnose (2 g). Exercise included two 60-min treadmill runs at 70 % of VO(2)max at 30 A degrees C after ingestion of glutamine (Gln) or placebo (Pla). Plasma levels of endotoxin and TNF-alpha, along with peripheral blood mononuclear cell (PBMC) protein expression of HSP70 and I kappa B alpha, were measured pre- and post-exercise and 2 and 4 h post-exercise. Permeability increased in the Pla trial compared to that at rest (0.06 A +/- 0.01 vs. 0.02 A +/- 0.018) and did not increase in the Gln trial. Plasma endotoxin was lower at the 4-h time point in the Gln vs. 4 h in the Pla (6.715 A +/- 0.046 pg/ml vs. 7.952 A +/- 1.11 pg/ml). TNF-alpha was lower 4 h post-exercise in the Gln vs. Pla (1.64 A +/- 0.09 pg/ml vs. 1.87 A +/- 0.12 pg/ml). PBMC expression of IkB alpha was higher 4 h post-exercise in the Gln vs. 4 h in the Pla (1.29 A +/- 0.43 vs. 0.8892 A +/- 0.040). HSP70 was higher pre-exercise and 2 h post-exercise in the Gln vs. Pla (1.35 A +/- 0.21 vs. 1.000 A +/- 0.000 and 1.65 A +/- 0.21 vs. 1.27 A +/- 0.40). Acute oral glutamine supplementation prevents an exercise-induced rise in intestinal permeability and suppresses NF-kappa B activation in peripheral blood mononuclear cells.
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