期刊
CELL STRESS & CHAPERONES
卷 13, 期 4, 页码 527-533出版社
SPRINGER
DOI: 10.1007/s12192-008-0040-6
关键词
CCT; Chaperonin; Real-time PCR; Scarless wound healing
类别
资金
- Allegheny Singer Research Institute
- Allegheny General Hospital
- Pittsburgh Tissue Engineering Institute (PTEI)
- K08 DE014780
- [DC 05659]
- [DC 04173]
Integumentary wound healing in early fetal life is regenerative and proceeds without scar formation. Expressomic analysis of this phenomenon by differential display has previously determined that the eta subunit of the cytosolic chaperonin containing T-complex polypeptide (CCT) is downregulated in the healing fetal wound milieu. We now report that no other CCT subunit shares this distinct pattern of gene regulation as determined by limiting dilution reverse transcriptase polymerase chain reaction (RT-PCR); all seven of the remaining CCT subunits demonstrate no change in messenger RNA (mRNA) expression in healing fetal wounds compared to unwounded control tissue. The alpha subunit, however, did evidence reduced message levels in healing adult wound tissue. We herein report on the cloning and sequence of the complementary DNA (cDNA) for rabbit CCT-alpha and confirm its wound specific decrease in adult tissues through quantitative real-time RT-PCR assay. We also confirm that quantitative evaluation of CCT-alpha and CCT-zeta mRNA expression shows no change in healing fetal wounds.
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