期刊
CELL STEM CELL
卷 8, 期 6, 页码 633-638出版社
CELL PRESS
DOI: 10.1016/j.stem.2011.05.001
关键词
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资金
- Core Research for Evolutional Science and Technology (CREST)
- Ministry of Education, Culture, Sports, Science, and Technology [S: 21229015]
- Ministry of Health, Labour and Welfare
- Funding Program for Next Generation World-Leading Researchers, Japan [LS094]
- Tokyo Biochemical Research Foundation
- Grants-in-Aid for Scientific Research [22130005, 21592014, 23800039, 21591644] Funding Source: KAKEN
Induced pluripotent stem cells (iPSCs) can be generated from differentiated human and mouse somatic cells using transcription factors such as Oct4, Sox2, Klf4, and c-Myc. It is possible to augment the reprogramming process with chemical compounds, but issues related to low reprogramming efficiencies and, with a number of protocols, residual vector sequences, remain to be resolved. We show here that it is possible to reprogram mouse and human cells to pluripotency by direct transfection of mature double-stranded microRNAs (miRNAs). Our approaches use a combination of mir-200c plus mir-302 s and mir-369 s family miRNAs. Because this reprogramming method does not require vector-based gene transfer, it holds significant potential for biomedical research and regenerative medicine.
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