期刊
CELL STEM CELL
卷 8, 期 1, 页码 106-118出版社
CELL PRESS
DOI: 10.1016/j.stem.2010.12.003
关键词
-
资金
- NIH/NICHD
- Hartwell Foundation
- CIRM [CL1-00502, RT1-01108, TR1-01250, RN2-00931-1]
- NIH [R21MH087925, R01 HL64387, P01 HL094374, R01 HL084642, P01 GM081719]
- Millipore Foundation
- Esther O'Keefe Foundation
- Edmond J. Safra foundation in Tel Aviv University
- Legacy stem cell research fund
- PEW Charitable Trust
- Ministry of Education, Science and Technology [SC2250]
- Ministerio de Ciencia e Innovacion of Spain [RYC-2007-01510, SAF2009-08588]
- MICINN Fundacion Cellex
- G. Harold and Leila Y. Mathers Charitable Foundation
- Sanofi-Aventis
- NHLBI [RC1HL100168]
- Israel Science Foundation [802/08]
- Australian Stem Cell Centre
- Victoria-California Stem Cell Alliance, CIRM [TR101250]
- Victoria-California Stem Cell Alliance, state government of Victoria, Australia [TR101250]
Genomic stability is critical for the clinical use of human embryonic and induced pluripotent stem cells. We performed high-resolution SNP (single-nucleotide polymorphism) analysis on 186 pluripotent and 119 nonpluripotent samples. We report a higher frequency of subchromosomal copy number variations in pluripotent samples compared to nonpluripotent samples, with variations enriched in specific genomic regions. The distribution of these variations differed between hESCs and hiPSCs, characterized by large numbers of duplications found in a few hESC samples and moderate numbers of deletions distributed across many hiPSC samples. For hiPSCs, the reprogramming process was associated with deletions of tumor-suppressor genes, whereas time in culture was associated with duplications of oncogenic genes. We also observed duplications that arose during a differentiation protocol. Our results illustrate the dynamic nature of genomic abnormalities in pluripotent stem cells and the need for frequent genomic monitoring to assure phenotypic stability and clinical safety.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据