期刊
APPLIED MAGNETIC RESONANCE
卷 23, 期 3-4, 页码 571-583出版社
SPRINGER WIEN
DOI: 10.1007/BF03166642
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The pulsed electron-electron double resonance (ELDOR)-detected nuclear magnetic resonance (NMR) technique has been applied to the investigation of the hyperfine structure of the oxidized radical Of Y-D tyrosine (Y-D*) and Mn2+ ion bound to the high-affinity site in photosystem II. The resulting ELDOR spectrum of Y-D* was found to correspond with the pulsed electron-nuclear double resonance (ENDOR) spectrum except for a slightly larger linewidth (by a factor of 1.7). The spectrum showed marked anisotropy and revealed three peaks which can be assigned to matrix protons, C-3 and -5 protons and one of the beta-protons in the tyrosine molecule. The results demonstrate that the pulsed ELDOR-detected NMR technique is applicable to the study of organic radicals in biological systems. The Mn2+ ion bound to the high-affinity site in photosystem II yielded well-resolved ELDOR signals spreading over +/-1000 MHz. The magnetic properties of the Mn2+ were characterized on the basis of the calculation of the ENDOR transitions and the anisotropy of the ELDOR spectrum.
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