期刊
CELL STEM CELL
卷 3, 期 4, 页码 391-401出版社
CELL PRESS
DOI: 10.1016/j.stem.2008.07.027
关键词
-
资金
- Netherlands Organization for Scientific Research (NWO) [TALENT 809.67.024]
- Japan Society for Promotion of Science
- CellCentric Ltd [TP/4/BIO/6/I/22020]
- Department for Innovation, Universities, and Skills (DIUS)
- Wellcome Trust [062801]
Embryonic stem cells (ESCs) are apparently homogeneous self-renewing cells, but we observed heterogeneous expression of Stella in ESCs, which is a marker of pluripotency and germ cells. Here we show that, whereas Stella-positive ESCs were like the inner cell mass (ICM), Stella-negative cells were like the epiblast cells. These states were interchangeable, which reflects the metastability and plasticity of ESCs. The established equilibrium was skewed reversibly in the absence of signals from feeder cells, which caused a marked shift toward an epiblast-like state, while trichostatin A, an inhibitor of histone deactelylase, restored Stella-positive population. The two populations also showed different histone modifications and striking functional differences, as judged by their potential for differentiation. The Stella-negative ESCs were more like the postimplantation epiblast-derived stem cells (EpiSCs), albeit the stella locus was repressed by DNA methylation in the latter, which signifies a robust epigenetic boundary between ESCs and EpiSCs.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据