期刊
CELL RESEARCH
卷 23, 期 12, 页码 1356-1368出版社
INST BIOCHEMISTRY & CELL BIOLOGY
DOI: 10.1038/cr.2013.145
关键词
hematopoietic stem cell; miR-142a-3p; irf7; zebrafish; mouse
类别
资金
- National Basic Research Program of China [2010CB945300, 2011CB943900, 2012CB945101]
- National Natural Science Foundation of China [31271570, 90919055]
- Strategic Priority Research Program of the Chinese Academy of Sciences [XDA01010110]
Previous studies on developmental hematopoiesis have mainly focused on signaling and transcription factors, while the appreciation of epigenetic regulation including that of microRNAs is recent. Here, we show that in zebrafish and mouse, miR-142-3p is specifically expressed in hematopoietic stem cells (HSCs). Knockdown of miR-142a3p in zebrafish led to a reduced population of HSCs in the aorta-gonad-mesonephros (AGM) region as well as T-cell defects in the thymus. Mechanistically, miR-142a-3p regulates HSC formation and differentiation through the repression of interferon regulatory factor 7 (irf7)-mediated inflammation signaling. Finally, we show that miR-142-3p is also involved in the development of HSCs in mouse AGM, suggesting that it has a highly conserved role in vertebrates. Together, these findings unveil the pivotal roles that miR-142a-3p plays in the formation and differentiation of HSCs by repressing irf7 signaling.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据